ABSTRACT
Major objective of the present work was to induce haploid plants in Cucumis melo var. conomon cv. Mudicode (Common name: Kani vellari). Ovules of Kani vellari plants were collected one day before anthesis and were cultured in vitro on MS medium supplemented with IAA, IBA, NAA, 2, 4-D, KN, BAP, TDZ and 2-iP (1.0, 2.0 and 5.0 μM) and BAP/TDZ/KN (1.0, 2.0 and 5.0 μM) in combination with IAA, IBA, NAA, 2, 4-D (1.0, 2.0 and 5.0 μM). The cultured ovules involved in callus induction and an optimum quantity of 93.15 mg of callus from cultured ovules was obtained on the MS medium fortified with 1 μM BAP + 2 μM NAA. The callus induced on medium supplemented with 1 μM BAP+2 μM NAA was subcultured on MS medium supplemented with 5 μM BAP was involved in shoot organogenesis and developed a maximum of 5.55 shoots. The rooting of the regenerated shoots was achieved on MS medium supplemented with 1 μM IAA. Cytological analysis of the root tips of regenerated plants confirmed their haploid nature.